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KMID : 1143420160090430854
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Public Health Weekly Report
2016 Volume.9 No. 43 p.854 ~ p.860
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Characterization of ‘hard-to-cultivate’ pathogens, Borrelia spp. and Leptospira spp. in Korea
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Jang Won-Jong
Lee Kyeong-Min
Hwang Kyu-Jam
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Abstract
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Background: Securing emerging and reemerging infectious pathogens is essential for medical vaccine development, pathogens diagnosis, and disease mechanism studies. Nagoya Protocol in 2014 triggered a great attention to microorganisms, and began to acknowledge them as biological resources. In this study, we isolated, characterized, and deposited 8 Leptospira spp. and 5 Borrelia spp. which were the wild animal- and tick-derived ‘hard-to-cultivate’ pathogens in South Korea.
Methodology: We captured rodents from various locations in South Korea where uncultured pathogen species are expected. We isolated the target species, and also reactivated previous storing pathogen samples, specifically Borrelia spp., from LN2 tanks to see their molecular characteristics. We employed UPGMA (Unweighted Pair Group Method with Arithmetic Mean) clustering technique to obtain a relationship of the pathogens based on genetic distance.
Results: We built a relationship for Leptospira spp. using LipL32 where 8 captured strains were included. The relationship showed that they are the most closely related to L. interrogans strain lai as previously known. A relationship of Borrelia spp. on 16s rDNA showed the strains captured in the same geographic location were closely related to at least two different species. We believe that the addition of our result to the depository such as National Culture Collection for Pathogens would be useful for future medical studies, including vaccine development, diagnostic marker searching, and the research of disease mechanisms.
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KEYWORD
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